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KMID : 0380619790110040283
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Korean Journal of Food Science and Technology
1979 Volume.11 No. 4 p.283 ~ p.290
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Production of Fructose from Jerusalem Artichoke Tubers By Enzymatic Hydrolysis
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변시명/Byun, Si Myung
남백희/김우연/Nahm, Baek Hie/Kim, Woo Yeon
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Abstract
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Partially purified β-fructosidase (inulase) from Kluyveromyces fragilis was immobilized on Tygon tube and aminoethyl-cellulose, respectively and both preparations were characterized. Silanization of Tygon tube in chloroform at 65℃ and treatment with 10 % glutaraldehyde were critical for the immobilization of inulase on Tygon tube, while 2 % glutaraldehyde was effective for the immobilization on aminoethyl-cellulose.
The derivative of Tygon tube showed 11.5 units of inulase activity per g of dried matrix with retention of 22.5 % of original activity against inulin, whereas one of aminoethyl-cellulose showed 39.3 units per g of dired matrix with 53.4 % of retention. Studies of enzyme stability, pH and temperature dependences, and K_m values are presented for inulase and invertase activities of both immobilized enzymes.
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